Specificity of antibodies to diphtheria toxin subunits in children with various forms of diphtheria infections

• Published in: Ukrains'kyi biokhimichnyi zhurnal (1999 ) Vol. 73; no. 6; p. 73
• Main Authors: Romaniuk, S I, Kolibo, D V, Kavun, E M, Radavskiĭ, Iu L, Tkachenko, T Iu, Kramarev, S A, Komissarenko, S V
• Format: Journal Article
• Language: Russian
• Published: Ukraine 01.11.2001
• Subjects: Antibodies, Bacterial - analysis; Antibody Specificity; Child; Diphtheria - immunology; Diphtheria Toxin - immunology; Diphtheria-Tetanus-Pertussis Vaccine - administration & dosage; Diphtheria-Tetanus-Pertussis Vaccine - immunology; Electrophoresis, Polyacrylamide Gel; Humans

In order to study the specificity of serum antibodies to separate subunits of diphtheria toxin, SDS-electrophoresis of diphtheria toxin preliminary disintegrated on the subunits via trypsin treatment was performed, followed by immunoblotting assay. 86 blood serum samples of children with diphtheria carriers of toxigenic and non-toxigenic strains of Corynebacterium diphtheriae as well as children with other infectious diseases similar to diphtheria in their clinical manifestation, and healthy ones immunized with DTP-vaccine were tested. A special computer program was written and applied for results processing and assumption. The data obtained showed that there were particular differences in frequency of predominating the antibodies to one or another subunit of diphtheria toxin among various groups of the children. We consider that the different specificity of antibodies of sick children and children-carriers is capable to predetermine the different course of infectious process.