Role of DNA-membrane attachment in repair of radiation damage in Micrococcus radiodurans

The unirradiated bacterial DNA assciated with the membrane is liberated into the cytoplasm after breakage of either a single or a double strand, resulting from X-ray action. During the reincubation period in growth-medium, the DNA is reassociated with the membrane. This phenomenon is very rapid and...

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Bibliographic Details
Published inInternational journal of radiation biology and related studies in physics, chemistry and medicine Vol. 27; no. 2; p. 157
Main Authors Dardalhon-Samsonoff, M, Rebeyrotte, N
Format Journal Article
LanguageFrench
Published England 01.02.1975
Subjects
Acetamides - pharmacology
Binding Sites
Chloramphenicol - pharmacology
DNA Repair
DNA, Bacterial
Membranes
Micrococcus - radiation effects
Radiation Effects
Radiation-Sensitizing Agents - pharmacology
Thymidine
Tritium
X-Rays
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Summary:The unirradiated bacterial DNA assciated with the membrane is liberated into the cytoplasm after breakage of either a single or a double strand, resulting from X-ray action. During the reincubation period in growth-medium, the DNA is reassociated with the membrane. This phenomenon is very rapid and occurs without increasing the molecular weight of DNA. The study of DNA-membrane complexes shows that the size of the DNA-associated membranous fragment differs according to the lysing technique employed, appearing as a change in the density of the complex. Chloramphenicol decreases reassociation, and iodoacetamide, a radiosensititzing agent, inhibits it completely.
ISSN:0020-7616