Practical Tips for Operating UHPLC Instruments and Columns

Traditional high-performance liquid chromatography (HPLC) method development simply required careful definition of column, mobile phase and detector conditions. However, for ultrahigh-pressure liquid chromatography (UHPLC), which offers very high column efficiency and narrow sample elution bands, th...

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Bibliographic Details
Published inLC GC North America Vol. 31; no. S4a; p. 28
Main Authors Henry, Richard A, Brandes, Hillel K, Nowlan, Daniel T, Best, John W
Format Trade Publication Article
LanguageEnglish
Published Monmouth Junction MultiMedia Healthcare Inc 01.04.2013
Subjects
Advantages
Atoms & subatomic particles
Bandwidths
Chromatography
Design
Efficiency
Particle size
Retention
Sensors
Velocity
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Summary:Traditional high-performance liquid chromatography (HPLC) method development simply required careful definition of column, mobile phase and detector conditions. However, for ultrahigh-pressure liquid chromatography (UHPLC), which offers very high column efficiency and narrow sample elution bands, the UHPLC instrument components must be carefully matched in performance with columns to achieve desired resolution. If UHPLC underperforms, it can be difficult to determine whether the instrument or column is the problem. This article will describe the design of modern UHPLC instruments and offer simple procedures to identify the causes of poor column and method performance. Modern textbooks do not yet adequately address the complex topic of UHPLC, therefore the reader is encouraged to collect all references to learn important, additional details. [PUBLICATION ABSTRACT]
ISSN:1527-5949
1939-1889