Maintenance of red blood cell integrity by AMP-activated protein kinase a1 catalytic subunit

AMP-activated protein kinase (AMPK) plays a pivotal role in regulating cellular energy metabolism. We previously showed that AMPKa1a/a mice develop moderate anemia associated with splenomegaly and high reticulocytosis. Here, we report that splenectomy of AMPKa1a/a mice worsened anemia supporting evi...

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Published inFEBS letters Vol. 584; no. 16; pp. 3667 - 3671
Main Authors etz, Marc, Guihard, Soizic, Leclerc, Jocelyne, Fauveau, Veronique, Couty, Jean-Pierre, Andris, Fabienne, Gaudry, Murielle, Andreelli, Fabrizio, Vaulont, Sophie, Viollet, Benoit
Format Journal Article
LanguageEnglish
Published 20.08.2010
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Summary:AMP-activated protein kinase (AMPK) plays a pivotal role in regulating cellular energy metabolism. We previously showed that AMPKa1a/a mice develop moderate anemia associated with splenomegaly and high reticulocytosis. Here, we report that splenectomy of AMPKa1a/a mice worsened anemia supporting evidence that AMPKa1a/a mice developed a compensatory response through extramedullary erythropoiesis in the spleen. Transplantation of bone marrow from AMPKa1a/a mice into wild-type recipients recapitulated the hematologic phenotype. Further, AMPKa1a/a red blood cells (RBC) showed less deformability in response to shear stress limiting their membrane flexibility. Thus, our results highlight the crucial role of AMPK to preserve RBC integrity.
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ISSN:0014-5793
DOI:10.1016/j.febslet.2010.07.041