Antioxidant Activity of Flavonoids Isolated from Vitex rotundifolia

The aim of this investigation was to evaluate antioxidant activity of crude extracts from the halophyte Vitex rotundifolia, their solvent fractions, and isolated compounds (1-3). Antioxidant capacity was determined by measuring DPPH radical, and authentic ONOO super(-) and ONOO super(-) generated fr...

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Bibliographic Details
Published inOcean and polar research Vol. 33; no. 3; pp. 255 - 263
Main Authors Kim, YA, Lee, JI, Hong, J W, Jung, ME, Seo, Y
Format Journal Article
LanguageKorean
Published 01.09.2011
Subjects
Marine
Vitex rotundifolia
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Summary:The aim of this investigation was to evaluate antioxidant activity of crude extracts from the halophyte Vitex rotundifolia, their solvent fractions, and isolated compounds (1-3). Antioxidant capacity was determined by measuring DPPH radical, and authentic ONOO super(-) and ONOO super(-) generated from 3-morpholinsydnonimine (SIN-1) in vitro as well as degree of occurrence of intracellular ROS, NO and GSH in mouse macrophage Raw 264.7 cells. From comparative analysis, MeOH extract, n-BuOH, and 85% aq. MeOH solvent fractions showed significant antioxidant effect in DPPH radical and ONOO super(-) assay systems. Activity-guided purification of n-BuOH and 85% aq. MeOH fractions led to the isolation of flavonoids 1-3. Among them, compound 1 exhibited excellent antioxidant effect in all bioassay systems tested. On the other hand, compounds 2 and 3 revealed potent inhibitory effect against ONOO super(-) generated from SIN-1, comparable with the positive control penicillamine.
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ISSN:1598-141X
2234-7313