Investigation of the interaction of repair DNA polymerase Delta b and autonomous 3' -> 5'-exonucleases TREX1 and TREX2

• Published in: Biology bulletin of the Russian Academy of Sciences Vol. 37; no. 5; pp. 464 - 470
• Main Authors: Belyakova, N V, Legina, O K, Ronzhina, N L, Shevelev, I V, Krutiakov, V M
• Format: Journal Article
• Language: English
• Published: 01.01.2010
• Subjects: DNA repair; DNA-directed DNA polymerase; Filtration; Proofreading; Pyroxylin; Western blotting
• ISSN: 1062-3590
• DOI: 10.1134/S1062359010050043

The possibility of interaction of recombinant proteins of human repair DNA polymerase Delta *b with proofreading 3' -> 5'-exonucleases TREX1 and TREX2 was investigated in vitro for the first time. The results of gel filtration analysis show the formation of a complex between 3' -> 5'-exonucleases mTREX1 and hTREX2 and DNA polymerase Delta *b. DNA polymerase activity is shown to increase four-fold in the presence of 3' -> 5'-exonuclease TREX2. The experiments with the use of immunodot and Western blot assays on the binding of DNA-polymerase Delta *b with 3' -> 5'-exonucleases TREX1 and TREX2 immobilized on a nitrocellulose membrane provided additional evidence on the direct association of the above proteins in complexes.