Recombinant Components of the EcoRII Restriction-Modification System: Restriction Endonuclease Can Interact with DNA.RNA Duplexes

To obtain recombinant restriction endonuclease (R) and methylase (M) of the EcoRII restriction-modification system, bacterial strains overproducing their functional hexahistidine derivatives were constructed. Active full-length R.EcoRII was produced only in cells that also expressed M.EcoRII from a...

Full description

Saved in:
Bibliographic Details
Published inMolecular biology (New York) Vol. 34; no. 6; pp. 913 - 920
Main Authors Babkina, O V, Evstafieva, A G, Chichkova, N V, Vartapetian, AB, Mueller, S, Baskunov, V B, Petrauskene, O V, Kochetkov, S N, Gromova, E S
Format Journal Article
LanguageEnglish
Published 01.01.2000
Online AccessGet full text

Cover

More Information
Summary:To obtain recombinant restriction endonuclease (R) and methylase (M) of the EcoRII restriction-modification system, bacterial strains overproducing their functional hexahistidine derivatives were constructed. Active full-length R.EcoRII was produced only in cells that also expressed M.EcoRII from a multicopy plasmid. Recombinant R.EcoRII bound with hybrid DNA.RNA duplexes.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
content type line 23
ObjectType-Feature-2
ISSN:0026-8933
1608-3245
DOI:10.1023/A:1026688028427