Recombinant Components of the EcoRII Restriction-Modification System: Restriction Endonuclease Can Interact with DNA.RNA Duplexes
To obtain recombinant restriction endonuclease (R) and methylase (M) of the EcoRII restriction-modification system, bacterial strains overproducing their functional hexahistidine derivatives were constructed. Active full-length R.EcoRII was produced only in cells that also expressed M.EcoRII from a...
Saved in:
Published in | Molecular biology (New York) Vol. 34; no. 6; pp. 913 - 920 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
01.01.2000
|
Online Access | Get full text |
Cover
Loading…
Summary: | To obtain recombinant restriction endonuclease (R) and methylase (M) of the EcoRII restriction-modification system, bacterial strains overproducing their functional hexahistidine derivatives were constructed. Active full-length R.EcoRII was produced only in cells that also expressed M.EcoRII from a multicopy plasmid. Recombinant R.EcoRII bound with hybrid DNA.RNA duplexes. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-2 |
ISSN: | 0026-8933 1608-3245 |
DOI: | 10.1023/A:1026688028427 |