Sensitive and Specific Phenotypic Assay for Metallo-{beta}-Lactamase Detection in Enterobacteria by Use of a Moxalactam Disk Supplemented with EDTA

Moxalactam is highly hydrolyzed by plasmid-mediated metallo-?-lactamases (MBLs), whereas it is poorly inactivated by serine-active carbapenemases. This study demonstrated that moxalactam resistance constituted an effective screen for MBL expression in enterobacteria, which could be confirmed, even i...

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Published inJournal of clinical microbiology Vol. 49; no. 7; p. 2667
Main Authors Pluquet, Emilie, Arlet, Guillaume, Bingen, Edouard, Drieux, Laurence, Mammeri, Hedi
Format Journal Article
LanguageEnglish
Published 01.07.2011
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Summary:Moxalactam is highly hydrolyzed by plasmid-mediated metallo-?-lactamases (MBLs), whereas it is poorly inactivated by serine-active carbapenemases. This study demonstrated that moxalactam resistance constituted an effective screen for MBL expression in enterobacteria, which could be confirmed, even in low-MBL-producing isolates, by a disk potentiation test using moxalactam and EDTA.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.00328-11