Immortalized cell lines for realatime analysis of circadian pacemaker and peripheral oscillator properties

In the mammalian circadian system, cell-autonomous clocks in the suprachiasmatic nuclei (SCN) are distinguished from those in other brain regions and peripheral tissues by the capacity to generate coordinated rhythms and drive oscillations in other cells. To further establish in vitromodels for dist...

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Published inThe European journal of neuroscience Vol. 33; no. 8; pp. 1533 - 1540
Main Authors Farnell, Yuhua F, Shende, Vikram R, Neuendorff, Nichole, Allen, Gregg C, Earnest, David J
Format Journal Article
LanguageEnglish
Published 01.04.2011
Subjects
Brain
Cell culture
Circadian rhythms
CLOCK protein
Fibroblasts
Fusion protein
Luminescence
Nervous system
Oscillators
Pacemakers
Period 2 protein
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Summary:In the mammalian circadian system, cell-autonomous clocks in the suprachiasmatic nuclei (SCN) are distinguished from those in other brain regions and peripheral tissues by the capacity to generate coordinated rhythms and drive oscillations in other cells. To further establish in vitromodels for distinguishing the functional properties of SCN and peripheral oscillators, we developed immortalized cell lines derived from fibroblasts and the SCN anlage of mPer2Lucknockin mice. Circadian rhythms in luminescence driven by the mPER2::LUC fusion protein were observed in cultures of mPer2
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ISSN:0953-816X
1460-9568
DOI:10.1111/j.1460-9568.2011.07629.x