Composition and biology activity of essential oils from leaves and stems of Rhodostemonodaphne parvifolia Madrinan (Lauraceae)

• Published in: Acta amazonica Vol. 40; no. 3; pp. 567 - 572
• Main Authors: Alcantara, J M, de Lima Yamaguchi, KK, de Andrade Silva, JR, da Veiga Junior, VF
• Format: Journal Article
• Language: Portuguese
• Published: 01.09.2010
• Subjects: Acetylcholinesterase; Lauraceae
• ISSN: 0044-5967

The essential oils of leaves and stems of Rhodostemonodaphne parvifolia collected in the Adolpho Ducke Forest Reserve were obtained by hydrodistillation and analyzed by GC-FID and GC-MS. Thirteen components were identified in the leaf oil, of which beta -caryophyllene was the major (41.30%). Fifty components were identified in the stem oil, among them the sesquiterpenes beta -caryophyllene (16.20%) and epi-cedrol (13.30%), together with the monoterpene linalool (15.40%). The essential oils were analyzed at chemical, DPPH free radical scavenging; and enzymatic bioassays, acetylcholinesterase inhibition. At antioxidant DPPH assays, the oils showed to be active at the qualitative assay but only weakly active at quantitative test (inactive at concentration lower than 1,000 mu g/mL). The inhibition of acetylcholinesterase was detected only in leaves essential oils. This paper describes for the first time the composition of these essential oils and their biological activities.Original Abstract: Os oleos essenciais das folhas e caules de Rhodostemonodaphne parvifolia coletada na Reserva Florestal Adolpho Ducke foram obtidos por hidrodestilacao e analisados por cromatografia em fase gasosa com detector de ionizacao de chama e espectrometria de massas. Treze constituintes foram identificados nas folhas, dos quais o beta -cariofileno foi o que apresentou maior percentual (41,30%). Nos caules foram identificados quinze compostos, com predominancia dos sesquiterpenos beta -cariofileno (16,20%) e epi-cedrol (13,30%) e do monoterpeno linalol (15,40%). Os oleos foram analisados em ensaios quimicos, de sequestro do radical livre DPPH, e enzimaticos, de inibicao da enzima acetilcolinesterase. Nos ensaios de sequestro de radicais livres, apesar de terem se mostrado ativos em ensaios qualitativos, os oleos essenciais apresentaram fraca atividade antioxidante no ensaio quantitativo (inativos em concentracao inferior a 1.000 mu g/mL). A inibicao da acetilcolinesterase so foi observada nos oleos essenciais das folhas. A composicao quimica e a atividade biologica destes oleos estao sendo relatadas pela primeira vez neste trabalho.