EVALUATION AMONG FOUR SEROLOGICAL TECHNIQUES OF BRUCELLA ABORTUS INFECTION IN BOVINES

Serum samples from 200 vaccinated adult bovine females from two herds were analyzed by buffered antigen acidified plate test (AAT) (Rose Bengal Plate Test), indirect enzyme-linked immunosorbent assay (ELISAI), 2-mercaptoethanol test (2-ME) and complement fixation test (FC). For ELISAI, the fixed val...

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Published inArquivos do Instituto Biológico (São Paulo) Vol. 76; no. 1; pp. 9 - 15
Main Authors Paulin, LMS, Andrade-Pacheco, WA, Castro, V, Federsoni, ISP
Format Journal Article
LanguageEnglish
Published 01.01.2009
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Summary:Serum samples from 200 vaccinated adult bovine females from two herds were analyzed by buffered antigen acidified plate test (AAT) (Rose Bengal Plate Test), indirect enzyme-linked immunosorbent assay (ELISAI), 2-mercaptoethanol test (2-ME) and complement fixation test (FC). For ELISAI, the fixed value of 45 percent positivity (PP) was used. Group A was composed of 100 animals, with description of reproductive disturbances compatible with brucellosis and reagent to the AAT. Group B was composed of 100 animals serologically free of B. abortus for at least two years. Additionally, all serum samples were tested using the AAT, the 2-ME and the FC to confirm negative status. The combination of two tests, FC and 2-ME was used as the gold standard. The relative sensitivity and specificity and the Kappa were calculated for each test. The result of kappa for 2-ME in relation to the FC and of the AAT and the ELISAI in relation to the gold standard was, respectively, 0.78, 0.86 And 0.84. The relative sensitivities (Sr) were, respectively, 84.1% (53/63), 100% (53/53) and 98.1% (52/53), and the relative specificities (Er) were 93.3% (111/119), 90.1% (100/111) and 90.1% (100/111). For comparison between the ELISAI and the AAT, there was obtained a Kappa of 0.91, Sr of 93% (93/100) and Er of 98% (98/100). Conclusions: 1 - The option of constituting the gold standardbased on at least two tests was the most suitable for this study; 2 - The ELISAI resulted in values of Sr and Er similar to the AAT. Therefore, the AAT and the ELISAI are good for screening in regard to the diagnosis of brucellosis.Original Abstract: O estudo objetivou comparar o desempenho do teste mercaptoetanol (2-ME) com o teste fixacao de complemento (FC) e os testes antigeno acidificado tamponado (AAT) e ELISA indireto (ELISAI) frente ao gold standard, para o sorodiagnostico da brucelose em bovinos. Tambem se comparou o ELISAI com o AAT. Foram processadas 200 amostras de soro de femeas bovinas adultas de dois rebanhos, denominados grupos A e B. O grupo A foi composto de 100 femeas vacinadas contra brucelose, com historico de problemas reprodutivos compativeis com a doenca e reatoras ao AAT. O grupo B foi composto de 100 femeas vacinadas e nao reatoras ao AAT por mais de dois anos. Todos os soros foram submetidos aos quatro testes. Foi empregado, como gold standard, a combinacao do FC com o 2-ME. O indice Kappa foi usado como medida de concordancia e seu resultado, para o 2-ME, o AAT e o ELISAI foi de, respectivamente, 0,78, 0,86 e 0,84. As sensibilidades relativas (Sr) foram de, respectivamente, 84,1%, 100% y 98,1% e as especificidades relativas (Er), 93,3%, 90,9% e 90,1%. Entre o ELISAI e o AAT, obteve-se Kappa de 0,91, Sr de 93% e Er de 98%. Conclusoes: 1-A opcao de se contituir o gold standard baseado em dois testes foi a mais adequada para este estudo; 2-Os valores de Sr e Er do ELISAI foram semelhantes aos do AAT. 3-O AAT e o ELISAI mostraram ser testes muito sensiveis e de boa especificidade e, portanto, adequados para serem usados como triagem no diagnostico da brucelose.
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ISSN:0020-3653