Development of Quercetin Biosensor Through Immobilizing Laccase in a Modified b-Cyclodextrin Matrix Containing Ag Nanoparticles in Ionic Liquid

A biosensor containing Ag nanoparticles in ionic liquid (IL) 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (BMI.Tf2N) and laccase (Lac) immobilized on -cyclodextrin modified with epichlorohydrin (-CDEpi) was developed for quercetin determination. Lac catalyses the oxidation of querce...

Full description

Saved in:
Bibliographic Details
Published inElectroanalysis (New York, N.Y.) Vol. 22; no. 12; pp. 1376 - 1385
Main Authors Franzoi, Ana Cristina, Vieira, Iolanda Cruz, Scheeren, Carla Weber, Dupont, Jairton
Format Journal Article
LanguageEnglish
Published 01.06.2010
Online AccessGet full text

Cover

More Information
Summary:A biosensor containing Ag nanoparticles in ionic liquid (IL) 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (BMI.Tf2N) and laccase (Lac) immobilized on -cyclodextrin modified with epichlorohydrin (-CDEpi) was developed for quercetin determination. Lac catalyses the oxidation of quercetin to quinone, which is then reduced on the biosensor surface and the resulting current was investigated by square-wave voltammetry. The -CDEpi support was analyzed by scanning electron microscopy and Ag-BMI.Tf2N by transmission electron microscopy and X-ray diffraction. The linear range (0.499-7.407 M) and low detection limit (0.037c0.004 M) show that the proposed biosensor is suitable for quercetin determination in real samples.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
content type line 23
ObjectType-Feature-2
ISSN:1040-0397
DOI:10.1002/elan.200900591