b2- and b3-, but not b1-adrenergic receptors are involved in osteogenesis of mouse mesenchymal stem cells via cAMP/PKA signaling

• Published in: Archives of biochemistry and biophysics Vol. 496; no. 2; pp. 77 - 83
• Main Authors: Li, Haifang, Fong, Chichun, Chen, Yao, Cai, Guoping, Yang, Mengsu
• Format: Journal Article
• Language: English
• Published: 15.04.2010
• ISSN: 0003-9861
• DOI: 10.1016/j.abb.2010.01.016

The osteogenic capacity of mesenchymal stem cells (MSCs) and the importance of b-adrenergic signals in bone formation and resorption have been well investigated. However, little is known about the development of b-adrenergic receptor (b-AR) systems and the role of b-adrenergic signals in osteogenic differentiation of MSCs, which is critically important in bone physiology and pharmacology. In this study, we demonstrated that both the mRNA and protein levels of b2- and b3-AR are up-regulated following osteogenesis of mouse MSCs. We also established that b-AR agonists negatively while antagonists positively affect MSC osteogenesis. Both b2- and b3-AR are involved in MSC osteogenesis, with b2-AR being dominant. The effect of b-ARs on MSC osteogenesis is partly mediated via the cAMP/PKA signaling. These findings suggest that MSC is also a target for b-adrenergic regulation and b-adrenergic signaling plays a role in MSC osteogenesis.