Micro 3D Culture System using Hyaluronan-Collagen Capsule for Skeletal Muscle-Derived Stem Cells
In order to hold non-adhesive type cells while maintaining cellular interactions and various autocrine/paracrine factors, a micro 3D culture system using Hyaluronan (HA)-type I collagen capsules was investigated as a possible scaffold for cell transplantation. Skeletal muscle-derived enzymatically e...
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Published in | The open tissue engineering and regenerative medicine journal Vol. 3; pp. 18 - 27 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
01.01.2010
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Online Access | Get full text |
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Summary: | In order to hold non-adhesive type cells while maintaining cellular interactions and various autocrine/paracrine factors, a micro 3D culture system using Hyaluronan (HA)-type I collagen capsules was investigated as a possible scaffold for cell transplantation. Skeletal muscle-derived enzymatically extracted cells, which include numerous non-adhesive type stem cells were cultured in conventional liquid DMEM with and without encapsulation in HA-collagen capsules, and cellular proliferation/differentiation were compared. Results indicate that encapsulation does not disturb any cellular proliferation/differentiation after 7 days of culture. Gradual increases in vascular endothelial growth factor are also confirmed in HA-collagen culture, which may be induced by slower diffusion of autocrine/paracrine factors in the capsule and may benefit cellular proliferation/differentiation. Cell-holding capacity of encapsulation was also tested by in vivo transplantation into wide-open muscle scars without fascia. Encapsulation significantly contributes to higher donor cell implantation ratio and damaged muscle mass recovery than that of non-capsulation. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-1 |
ISSN: | 1875-0435 |
DOI: | 10.2174/1875043501003010018 |