i\In vitro\-i\ anti-HSV-2 activity and mechanism of action of proanthocyanidin A-1 from\+i\ Vaccinium vitis-idaea.\-i

• Published in: Journal of the science of food and agriculture Vol. 85; no. 1; pp. 10 - 15.
• Main Authors: Cheng, Hua-Yew, Lin, Ta-Chen, Yang, Chien-Min, Shieh, Den-En, Lin, Chun-Ching
• Format: Journal Article
• Language: English
• Published: 15.01.2005
• ISSN: 0022-5142
• DOI: 10.1002/jsfa.1958

The aim of this study was to investigate the in vitro anti-HSV-2 activity and mechanism of action of proanthocyanidin A-1, a compound isolated from Vaccinium vitis-idaea Linn (Ericaceae), the lingonberry. The results demonstrated that proanthocyanidin A-1 exhibited anti-HSV-2 activity. The IC(sub 50) value for the XTT assay was 73.3 +or- 14.5 *mM and the IC(sub 50) and IC(sub 90) values for the plaque reduction assay were 41.9 +or- 2.0 and 62.8 +or- 6.3 *mM respectively. Proanthocyanidin A-1 showed no cell cytotoxic effect at concentrations that blocked HSV-2 infection, with a CC(sub 50) value of 282.1 +or- 27.5 *mM. The mechanism studies demonstrated that proanthocyanidin A-1 did not reduce viral infectivity but inhibited viral attachment and penetration and affected the late stage(s) of HSV-2 infection. It was concluded that proanthocyanidin A-1 suppressed HSV-2 infection through many modes of action and thus merits further investigation.