Efficient purification and morphology characterization of paclitaxel from cell cultures of\+i\Taxus chinensis.\-i

Paclitaxel was purified from cell cultures of Taxus chinensis by a combination of extraction, low-pressure chromatography, precipitation and HPLC. A crude extract (purity 6.9%) was obtained by methanol extraction of plant cell cultures, followed by liquid-liquid extraction using dichloromethane. The...

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Published inJournal of chemical technology and biotechnology (1986) Vol. 79; no. 10; pp. 1162 - 1168.
Main Authors Pyo, Sang-Hyun, Kim, Moon-Suk, Cho, Jin-Suk, Song, Bong-Kyu, Han, Byung-Hee, Choi, Ho-Joon
Format Journal Article
LanguageEnglish
Published 01.10.2004
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Summary:Paclitaxel was purified from cell cultures of Taxus chinensis by a combination of extraction, low-pressure chromatography, precipitation and HPLC. A crude extract (purity 6.9%) was obtained by methanol extraction of plant cell cultures, followed by liquid-liquid extraction using dichloromethane. The extract was purified to greater than 32% with a 97% step yield by low-pressure chromatography. After acetone/pentane precipitation, the resulting purity and step yield were 75.8% and 97.4%, respectively. High performance liquid chromatography steps, which were composed of an HPLC step with column-packed ODS and an HPLC step with column-packed silica, were applied to give over 99.5% purity with high yield. Amorphous paclitaxel with a fine particle size, which has a solubility advantage compared with the stable crystalline form, was obtained by dissolving in dichloromethane, followed by spray-drying.
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SourceType-Scholarly Journals-1
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ISSN:0268-2575
DOI:10.1002/jctb.1111