Effect of hot water extract of Korean ginseng on neuroblastoma cell parthanatos

• Published in: Nan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 40; no. 9; pp. 1313 - 1318
• Main Authors: Guo, Yuanbo, Zhang, Dengwen, Long, Ruichun, Shan, Songgui, Sun, Qiang, Cai, Bin, Wang, Sheng
• Format: Journal Article
• Language: Chinese
• Published: 30.09.2020
• ISSN: 1673-4254
• DOI: 10.12122/j.issn.1673-4254.2020.09.14

OBJECTIVETo explore the effect of pretreatment of neuroblastoma cells with hot water extract of Korean ginseng on MNNG-induced parthanatos and its mechanism. METHODSNeuroblastoma SH-SY5Y cells were pretreated with 1 mg/L hot water extract of Korean ginseng before induction with 250 μmol/L MNNG for 1 h or 4 h. CCK-8 and cell flow cytometry were used to detect cell survival rate. Western blotting was used to detect the changes in poly(ADP-ribose) (PAR) expression in the treated cells. Immunofluorescence assay was used to detect nuclear distribution of apoptosis-inducing factor (AIF), and flow cytometry was used to detect the level of reactive oxygen species (ROS) in the cells. RESULTSCompared with the blank control cells, MNNG-treated SH-SY5Y cells showed significantly decreased survival rate as the concentration of MNNG and the stimulation time increased (P < 0.05). Stimulation with MNNG also resulted in significantly increased expression of PAR protein in the cells (P < 0.05). Pretreatment of the cells with h