The introduction of the 9a-hydroxy group into androst-4-en-3,17-dione using a new actinobacterium strain

A 9a-hydrolase activity of a new actinobacterium strain identified as Rhodococcus erythropolis based on the analysis of a 16S rRNA gene sequence (1417 nucleotides) towards androst-4-en-3,17-dione (AD) was studied. In the presence of glucose in the medium, this strain completely transformed AD (4-20...

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Published inApplied biochemistry and microbiology Vol. 45; no. 4; pp. 395 - 400
Main Authors Rodina, N V, Andryushina, V A, Stytsenko, T S, Turova, T P, Baslerov, R V, Panteleeva, AN, Voishvillo, N E
Format Journal Article
LanguageEnglish
Published 01.07.2009
Subjects
Rhodococcus erythropolis
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Summary:A 9a-hydrolase activity of a new actinobacterium strain identified as Rhodococcus erythropolis based on the analysis of a 16S rRNA gene sequence (1417 nucleotides) towards androst-4-en-3,17-dione (AD) was studied. In the presence of glucose in the medium, this strain completely transformed AD (4-20 g/l) into 9a-hydroxy-AD over 20-48 h. This culture was able to grow and perform AD 9a-hydroxylation at a concentration of dimethyl formamide up to 9%. Crystalline 9a-hydroxy-AD was isolated with a yield of over 90%.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ObjectType-Feature-1
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683809040085