A Plant Ca super(2+) Pump, ACA2, Relieves Salt Hypersensitivity in Yeast: MODULATION OF CYTOSOLIC CALCIUM SIGNATURE AND ACTIVATION OF ADAPTIVE Na super(+) HOMEOSTASIS

• Published in: The Journal of biological chemistry Vol. 283; no. 6; pp. 3497 - 3506
• Main Authors: Anil, Veena S, Rajkumar, Premraj, Kumar, Pavan, Mathew, M K
• Format: Journal Article
• Language: English
• Published: 08.02.2008
• Subjects: Arabidopsis thaliana
• ISSN: 0021-9258; 1083-351X

Stress responses in both plants and yeast utilize calcium-mediated signaling. A yeast strain, K616, which lacks Ca super(2+) pumps, requires micromolar Ca super(2+) for growth. In medium containing 100 mu M Ca super(2+), K616 can withstand osmotic stress (750 mM sorbitol) and ionic stress (300 mM KCl) but not hypersodic stress (300 mM NaCl). Heterologous expression of the endoplasmic reticulum-located Arabidopsis thaliana Ca super(2+)-ATPase, ACA2, permits K616 to grow under NaCl stress even in Ca super(2+)-depleted medium. All stresses tested generated transient elevation of cytosolic Ca super(2+) in wild type yeast, K601, whereas NaCl alone induced prolonged elevation of cytosolic Ca super(2+) in K616. Both the Ca super(2+) transient and survival of cultures subjected to NaCl stress was similar for the ACA2 transformant and K601. However, whereas K601 maintained low cytosolic Na super(+) predominantly by pumping it out across the plasma membrane, the transformant sequestered Na super(+) in internal organelles. This sequestration requires the presence of an endomembrane Na super(+)/H super(+)-antiporter, NHX1, which does not play a significant role in salt tolerance of wild type yeast except at acidic pH. Transcript levels of the plasma membrane Na super(+)-ATPase, ENA1, were strongly induced only in K601, whereas NHX1 was strongly induced in both K601 and the ACA2 transformant. The calmodulin kinase inhibitor KN62 significantly reduced the salt tolerance of the ACA2 transformant and the transcriptional induction of NHX1. Thus, the heterologous expression of a plant endomembrane Ca super(2+) pump results in the rapid depletion of cytosolic Ca super(2+) and the activation of an alternate mechanism for surviving saline stress.