Evidence for 1,25-Dihydroxyvitamin D sub(3)-independent Transactivation by the Vitamin D Receptor: UNCOUPLING THE RECEPTOR AND LIGAND IN KERATINOCYTES

• Published in: The Journal of biological chemistry Vol. 282; no. 15; pp. 10953 - 10962
• Main Authors: Ellison, Tara I, Eckert, Richard L, MacDonald, Paul N
• Format: Journal Article
• Language: English
• Published: 01.04.2007
• ISSN: 0021-9258; 1083-351X

The vitamin D endocrine system plays critical although poorly understood roles in skin. Vitamin D receptor (VDR) knock-out (VDRKO) mice have defects in hair follicle cycling and keratinocyte proliferation leading to epidermal thickening, dermal cyst formation, and alopecia. Surprisingly, skin defects are not apparent in mice lacking 25-hydroxyvitamin D 1 alpha -hydroxylase, the enzyme required for 1,25-dihydroxyvitamin D sub(3) (1,25(OH) sub(2)D sub(3)) hormone biosynthesis. These disparate phenotypes indicate that VDR effects in skin are independent of the 1,25(OH) sub(2)D sub(3) ligand. However, cellular or molecular data supporting this hypothesis are lacking. Here, we show transcriptional activation of the vitamin D-responsive 24-hydroxylase promoter by VDR in primary keratinocytes that is independent of the 1,25(OH) sub(2)D sub(3) ligand. This activity required functional vitamin D-responsive promoter elements as well as an intact VDR DNA binding domain and thus could not be distinguished from 1,25(OH) sub(2)D sub(3)-dependent VDR transactivation. The 1,25(OH) sub(2)D sub(3)-independent activation of VDR was also observed in keratinocytes from 1 alpha -hydroxylase knock-out mice, indicating that it is not due to endogenous 1,25(OH) sub(2)D sub(3) production. Mammalian two-hybrid studies showed strong, 1,25(OH) sub(2)D sub(3)-independent interaction between VDR and retinoid X receptors in primary keratinocytes, indicating that enhanced heterodimerization of these receptors was involved. Indeed, this 1,25(OH) sub(2)D sub(3)-independent VDR-RXR heterodimerization was sufficient to drive transactivation by VDR(L233S), an inactive ligand binding mutant of VDR that was previously shown to rescue the skin phenotype of VDR null mice. Cumulatively, these studies support the concept that transactivation by VDR in keratinocytes may be uncoupled from the 1,25(OH) sub(2)D sub(3) ligand.