Depletion of CD8 alpha super(+) lymphocytes attenuates CCL28 expression in villus epithelia in rats

• Published in: Immunology letters Vol. 124; no. 1; pp. 50 - 54
• Main Authors: Lee, J S, Kamada, S, Takami, Y, Oka, K, Ochiai, Y, Iwaya, H, Hara, H, Ishizuka, S
• Format: Journal Article
• Language: English
• Published: 14.05.2009
• ISSN: 0165-2478
• DOI: 10.1016/j.imlet.2009.04.004

To examine the involvement of CD8 alpha super(+) intraepithelial lymphocytes (IELs) in chemokine expression by villus epithelial cells, villus and crypt fractions were collected by mechanical isolation using a chelating buffer and specific antibodies in CD8 alpha super(+) cell-depleted rats. A larger population of CD8 alpha super(+) cells was observed by histochemical evaluation in villus epithelia than in crypt epithelia in rat small intestine, and CCL9 and CCL28 expression was higher in the crypt fraction than in the villus fraction. The mRNA expression of CCL28 in villus fractions isolated from rat small intestinal mucosa was significantly reduced compared to that of CCL9, and was accompanied by CD8 alpha depletion. Using a combined histochemical and flow cytometric approach, CD8 alpha alpha super(+) cells were detected in the intraepithelial region of the villus epithelium. Thus, CCL28 expression in villus epithelial cells is partially supported by CD8 alpha alpha super(+) cells, and CD8 alpha super(+) IELs are involved in CCL28 expression.