Prediction of Volatile Anesthetic Binding Sites in Proteins
Computational methods designed to predict and visualize ligand protein binding interactions were used to characterize volatile anesthetic (VA) binding sites and unoccupied pockets within the known structures of VAs bound to serum albumin, luciferase, and apoferritin. We found that both the number of...
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Published in | Biophysical journal Vol. 91; no. 9; pp. 3405 - 3414 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
01.11.2006
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Online Access | Get full text |
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Summary: | Computational methods designed to predict and visualize ligand protein binding interactions were used to characterize volatile anesthetic (VA) binding sites and unoccupied pockets within the known structures of VAs bound to serum albumin, luciferase, and apoferritin. We found that both the number of protein atoms and methyl hydrogen, which are within similar to 8 AA of a potential ligand binding site, are significantly greater in protein pockets where VAs bind. This computational approach was applied to structures of calmodulin (CaM), which have not been determined in complex with a VA. It predicted that VAs bind to [Ca super(2+)] sub(4)-CaM, but not to apo-CaM, which we confirmed with isothermal titration calorimetry. The VA binding sites predicted for the structures of [Ca super(2+)] sub(4)-CaM are located in hydrophobic pockets that form when the Ca super(2+) binding sites in CaM are saturated. The binding of VAs to these hydrophobic pockets is supported by evidence that halothane predominantly makes contact with aliphatic resonances in [Ca super(2+)] sub(4)-CaM (nuclear Overhauser effect) and increases the Ca super(2+) affinity of CaM (fluorescence spectroscopy). Our computational analysis and experiments indicate that binding of VA to proteins is consistent with the hydrophobic effect and the Meyer-Overton rule. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-2 |
ISSN: | 0006-3495 |
DOI: | 10.1529/biophysj.l06.082586 |