A Uniform Genomic Minor Histocompatibility Antigen Typing Methodology and Database Designed to Facilitate Clinical Applications

• Published in: PloS one Vol. 1; no. 1
• Main Authors: Spierings, Eric, Drabbels, Jos, Hendriks, Matthijs, Pool, Jos, Spruyt-Gerritse, Marijke, Claas, Frans, Goulmy, Els
• Format: Journal Article
• Language: English
• Published: 01.01.2006
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0000042.

Minor Histocompatibility x28; Hx29; antigen mismatches significantly influence the outcome of HLA-matched allogeneic stem cell transplantation. The molecular identification of human H antigens is increasing rapidly. In parallel, clinical application of minor H antigen typing has gained interest. So far, relevant and simple tools to analyze the minor H antigens in a quick and reliable way are lacking. Methodology and Findings We developed a uniform PCR with sequence-specific primers x28; PCR-SSPx29; for 10 different autosomal minor H antigens and H-Y. This genomic minor H antigen typing methodology allows easy incorporation in the routine HLA typing procedures. DNA from previously typed EBV-LCL was used to validate the methodology. To facilitate easy interpretation for clinical purposes, a minor H database named dbMinor x28.*