Isolation of Salmonella enterica subspecies enterica serovar Paratyphi B dT super(+), or Salmonella Java, from Indonesia and alteration of the d-tartrate fermentation phenotype by disrupting the ORF STM 3356

• Published in: Journal of medical microbiology Vol. 55; no. 12; pp. 1661 - 1665
• Main Authors: Han, KH, Choi, SY, Lee, J H, Lee, H, Shin, E H, Agtini, MD, von Seidlein, L, Ochiai, R L, Clemens, J D, Wain, J, Hahn, J-S, Lee, B K, Song, M, Chun, J, Kim, D W
• Format: Journal Article
• Language: English
• Published: 01.12.2006
• Subjects: Paratyphis; Salmonella enterica; Indonesia
• ISSN: 0022-2615
• DOI: 10.1099/jmm.0.46792-0

Salmonella enterica subspecies enterica serovar Paratyphi B [O1,4,(5),12: Hb: 1,2] can cause either an enteric fever (paratyphoid fever) or self-limiting gastroenteritis in humans. The d-tartrate non-fermenting variant S. enterica subsp. enterica serovar Paratyphi B dT super(-) (S. Paratyphi B) is the causative agent of paratyphoid fever, and the d-tartrate fermenting variant S. enterica subsp. enterica serovar Paratyphi B dT super(+) (S. Paratyphi B dT super(+); formerly called Salmonella Java) causes gastroenteritis. S. Java is currently recognized as an emerging problem worldwide. Twelve dT super(+) S. Java isolates were collected in Indonesia between 2000 and 2002. One-third of them contained Salmonella genomic island 1 (SGI1), which gives the multidrug-resistant phenotype to the bacteria. In this study, a PCR-based method to detect a single nucleotide difference responsible for the inability to ferment d-tartrate, reported elsewhere, was validated. The d-tartrate fermenting phenotype of S. Java was converted to the non-fermenting phenotype by the disruption of the ORF STM 3356, and the d-tartrate non-fermenting phenotype of the ORF STM 3356-disrupted strain and the dT similar to reference strain was changed to the dT super(+) phenotype by complementing ORF STM 3356 in trans. The results show that the dT super(+) phenotype requires a functional product encoded by STM 3356, and support the use of the PCR-based discrimination method for S. Paratyphi B and S. Java as the standard differentiation method.