HPV16 DNA in Historically Confirmed Tumour-free Neck Lymph Nodes of Head and Neck Cancers

• Published in: Anticancer research Vol. 26; no. 1B; pp. 663 - 670
• Main Authors: Hoffmann, M, Orlamuender, A, Sucher, J, Gottschlich, S, Goeroegh, T, Fazel, A, Ambrosch, P, Rittgen, W, Schwarz, E, Kahn, T
• Format: Journal Article
• Language: English
• Published: 01.02.2006
• Subjects: Human papilloma virus 16; Human papillomavirus; Human papillomavirus 16
• ISSN: 0250-7005

Human papillomavirus (HPV) has been demonstrated in lymph node neck metastases (NM) of HPV-positive squamous cell carcinomas of the head and neck (HNSCC), underscoring the possible role of HPV for HNSCC progression. Reports on HPV infections in histopathologically tumour-free lymph-nodes of the SCC of the uterine cervix developing higher rates of lymph-node metastases and recurrences later in the survey of the patients was the starting point of the present study. The presence of HPV-DNA in primary tumours (PT, n=45), NM (n=45) and histologically confirmed tumour-free neck lymph-nodes (LN, n=102) of HNSCC from 60 patients was analysed by PCR and Southern blot hybridisation. A highly positive correlation of simultaneous HPV-DNA detection in PT and NM was demonstrated. In the case of HPV-positivity of PT and/or NM [24/60 cases (40%)], 11/24 (45.8%) LN contained HPV-DNA, as well. Accepting HPV demonstration as a marker for the presence of micro-metastasis, HPV analysis would result in an upstaging of the N category in 4 out of these 11 patients. Considering the high agreement of HPV-DNA detection in PT and simultaneous HPV-DNA demonstration in the draining NM corroborating the monoclonal character of the tumour cells, the HPV-DNA presence in LN seems to be indicative of micro-metastasis in these lymph nodes. Thus, HPV analysis might be another powerful tool for the definition of the N-status of HPV-positive HNSCC.