Skim milk enhances the preservation of thawed -80 super(o)C bacterial stocks

The results from bacterial strain recovery efforts following hurricanes Katrina and Rita are reported. Over 90% of strains frozen in 10% skim milk were recovered whereas various recovery rates were observed for glycerol-stored stocks (56% and 94% of Escherichia coli, depending upon the laboratory)....

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Published inJournal of microbiological methods Vol. 75; no. 1; pp. 135 - 138
Main Authors Cody, W L, Wilson, J W, Hendrixson, DR, McIver, K S, Hagman, KE, Ott, C M, Nickerson, CA, Schurr, MJ
Format Journal Article
LanguageEnglish
Published 01.09.2008
Subjects
Borrelia burgdorferi
Campylobacter jejuni
Escherichia coli
Pseudomonas aeruginosa
Salmonella enterica
Streptococcus pyogenes
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Summary:The results from bacterial strain recovery efforts following hurricanes Katrina and Rita are reported. Over 90% of strains frozen in 10% skim milk were recovered whereas various recovery rates were observed for glycerol-stored stocks (56% and 94% of Escherichia coli, depending upon the laboratory). These observations led to a viability comparison of Streptococcus pyogenes, Campylobacter jejuni, Borrelia burgdorferi, Salmonella enterica subsp. Typhimurium, Pseudomonas aeruginosa and E. coli strains stored in glycerol or skim milk. In all bacteria examined, 10% skim milk resulted in significantly longer viability after thawing than 15% glycerol solutions currently used in most laboratories.
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ISSN:0167-7012
DOI:10.1016/j.mimet.2008.05.006