Original article: In vitro antiseptic properties of an ammonium compound combined with denture base acrylic resin

• Published in: Gerodontology Vol. 23; no. 2; pp. 111 - 116
• Main Authors: Pesci-Bardon, C, Fosse, T, Serre, D, Madinier, I
• Format: Journal Article
• Language: English
• Published: 01.06.2006
• Subjects: Candida albicans; Enterobacteriaceae; Escherichia coli; Pseudomonadaceae; Pseudomonas aeruginosa; Staphylococcus aureus
• ISSN: 0734-0664; 1741-2358
• DOI: 10.1111/j.1741-2358.2006.00088.x

Background:Denture base acrylic resin is easily colonised by oral endogenous bacteria and Candida spp., and eventually by extra-oral species such as Staphylococcus spp., Pseudomonadaceae or members of Enterobacteriaceae. This microbial reservoir can be responsive for denture related stomatitis and aspiration pneumonia, a life-threatening infection especially in geriatric patients. However, the oral and denture hygiene of dependant elderly individuals is extremely poor. Objective:This in vitro study aimed to determine the per cent of a quaternary ammonium compound heat-polymerised in acrylic resin necessary to obtain denture base displaying antiseptic properties. Design:Acrylic resin discs containing 2-50% ammonium polymer (Poly 202063A; 0% control) were soaked in artificial saliva for 4 weeks. Resin discs were incubated for 24 hours with Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa [37 degree C, brain-heart infusion (BHI) broth and phosphate-buffered saline (PBS) buffer] and Candida albicans (30 degree C, Schaedler broth), in 15 ml (168 discs) and 600 mu l (168 discs) of inoculum. Microbial growth was verified at t 0 hours and t 24 hours. Data were recorded as the mean of three colony forming unit (CFU) numerations. The borderline of antimicrobial effect was determined at 0.1% viable cells. Results:In 600 mu l of PBS inoculum, resin specimens had a bactericidal effect (E. coli and S. aureus: 2%; P. aeruginosa: 10%) and a fungicidal effect (C. albicans: 50%). Long-term stability and toxicity in vivo studies are now required. Conclusion:A 2% quaternary ammonium compound polymerised with a denture acrylic resin displayed antiseptic properties after a 4-week soaking period in artificial saliva. Such antiseptic denture base could help geriatric patients to improve their oral health.