Sequestration of a fluorinated analog of 2,4-dichlorophenol and metabolic products by L. minor as evidenced by super(1) super(9)F NMR

• Published in: Environmental pollution (1987) Vol. 145; no. 3; pp. 708 - 714
• Main Authors: Tront, J M, Saunders, F M
• Format: Journal Article
• Language: English
• Published: 01.02.2007
• ISSN: 0269-7491
• DOI: 10.1016/j.envpol.2006.05.039

Fate of halogenated phenols in plants was investigated using nuclear magnetic resonance (NMR) to identify and quantify contaminants and their metabolites. Metabolites of 4-chloro-2-fluorophenol (4-Cl-2-FP), as well as the parent compound, were detected in acetonitrile extracts using super(1) super(9)F NMR after various exposure periods. Several fluorinated metabolites with chemical shifts 3.5ppm from the parent compound were present in plant extracts. Metabolites isolated in extracts were tentatively identified as fluorinated-chlorophenol conjugates through examination of signal-splitting patterns and relative chemical shifts. Signal intensity was used to quantify contaminant and metabolite accumulation within plant tissues. The quantity of 4-Cl-2-F metabolites increased with time and mass balance closures of 90-110% were achieved. In addition, solid phase super(1) super(9)F NMR was used to identify 4-Cl-2-FP which was chemically bound to plant material. This work used super(1) super(9)F NMR for developing a time series description of contaminant accumulation and transformation in aquatic plant systems.