A Screen for Schizosaccharomyces pombe Mutants Defective in Rereplication Identifies New Alleles of rad4 super(+), cut9 super(+) and psf2 super(+)

Fission yeast mutants defective in DNA replication have widely varying morphological phenotypes. We designed a screen for temperature-sensitive mutants defective in the process of replication regardless of morphology by isolating strains unable to rereplicate their DNA in the absence of cyclin B (Cd...

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Bibliographic Details
Published inGenetics (Austin) Vol. 169; no. 1
Main Authors Gomez, Eliana B, Angeles, Vanessa T, sburg, Susan L
Format Journal Article
LanguageEnglish
Published 01.01.2005
Subjects
Schizosaccharomyces pombe
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Summary:Fission yeast mutants defective in DNA replication have widely varying morphological phenotypes. We designed a screen for temperature-sensitive mutants defective in the process of replication regardless of morphology by isolating strains unable to rereplicate their DNA in the absence of cyclin B (Cdc13). Of the 42 rereplication-defective mutants analyzed, we were able to clone complementing plasmids for 10. This screen identified new alleles of the APC subunit cut9 super(+) the initiation/checkpoint factor rad4 super(+)cut5 super(+) and the first mutant allele of psf2 super(+) a subunit of the novel GINS replication complex. Other genes identified are likely to play general roles in gene expression and protein localization.
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ISSN:0016-6731
DOI:10.1534/genetics.104.034231