(P 183) Generation of a Novel Sca-1 super(pos) Lin super(neg) Marine Mesenchymal Stem Cell Immortalized Line for Tissue Engineering Applications

• Published in: Tissue engineering. Part A Vol. 14; no. 5; p. 856
• Main Authors: te, G, Franzese, O, Pagliari, S, Pagliari, F, Fiaccavento, R, Carotenuto, F, Laudisi, A, Bonmassar, E, Minieri, M, Traversa, E, Di Nardo, P
• Format: Journal Article
• Language: English
• Published: 01.05.2008
• ISSN: 1937-3341; 1937-335X

Tissue engineering techniques rely on adult stem cells as a promising source of multipotent progenitors to repair damaged tissues and organs. In this context, the search for the ideal candidate for stem cell therapy is still ongoing and further in vitro investigations are required. Unfortunately, ex vivo-derived progenitor cells display high donor variability and limited lifespan in culture, proceeding to senescence within few passages. Recently, a unique Sca-1 super(pos)Lin super(neg) mesenchymal stem cell line (Sca-1 mTERT-MSC) was generated by mTERT ectopic transduction of bone marrow purified Sca-1 super(pos) MSC. This cell line, obtained by serial passages of single cell cloning, displays spindle-shaped morphology and continuously expresses mesenchymal stem cell markers (Sca-1, c-kit, Nanog, Nestin, Nucleostemin), while retaining the ability to differentiate towards osteogenic, adipogenic and myogenic lineages in standard culture conditions. Moreover, TERT-transduced MSC show enhanced resistance to H sub(2)O sub(2)-induced apoptosis similarly to early passage cells. Finally, when cultured onto three-dimensional biocompatible scaffolds, Sca-1 mTERT-MSC line displays the ability to maintain the typical stem cell phenotype and multipotency, thus suggesting its utilization as a powerful tool for engraftment and in vitro differentiation studies.