Ligand-Conjugated Quantum Dots Monitor Antigen Uptake and Processing by Dendritic Cells

The dendritic cell (DC) specific pathogen-uptake receptor (DC-SIGN) internalizes antigens for degradation and presentation onto MHC molecules. At the cell membrane, DC-SIGN forms nanoclusters that facilitate virus capture. However, internalized viruses, such as HIV-1, escape degradation. Here, we ex...

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Bibliographic Details
Published inNano letters Vol. 7; no. 4; pp. 970 - 977
Main Authors Cambi, A, Lidke, D S, Arndt-Jovin, D J, Figdor, C G, Jovin, T M
Format Journal Article
LanguageEnglish
Published 01.04.2007
Subjects
Human immunodeficiency virus 1
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Summary:The dendritic cell (DC) specific pathogen-uptake receptor (DC-SIGN) internalizes antigens for degradation and presentation onto MHC molecules. At the cell membrane, DC-SIGN forms nanoclusters that facilitate virus capture. However, internalized viruses, such as HIV-1, escape degradation. Here, we exploit ligand-conjugated, virus-sized, highly photostable quantum dots (QDs) to monitor in living cells antigen binding, entry, and trafficking. The antigen-coated QDs specific uptake and persistence in live DCs open the possibility for tracking antigen-presenting cells in vivo.
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ISSN:1530-6984
DOI:10.1021/n10700503