Radio-iodination of Monoclonal Antibody Using Potassium [ super(125)I]-(4-isothiocyanatobenzylammonio)-iodo-decahydro-closo- dodecab orate (Iodo-DABI)

Negatively-charged polyhedral boron clusters can be easily halogenated with highly stable boronhalogen bonds and are promising in radionuclide diagnostics and cancer therapy. The radio-iodination conditions for the closo-dodecaborate anion and for the conjugation of its labeled isothiocyanatobenzyla...

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Published inAnticancer research Vol. 26; no. 2A; pp. 1217 - 1224
Main Authors Orlova, A, Bruskin, A, Sivaev, I, Sjoeberg, S, Lundqvist, H, Tolmachev, V
Format Journal Article
LanguageEnglish
Published 01.04.2006
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Summary:Negatively-charged polyhedral boron clusters can be easily halogenated with highly stable boronhalogen bonds and are promising in radionuclide diagnostics and cancer therapy. The radio-iodination conditions for the closo-dodecaborate anion and for the conjugation of its labeled isothiocyanatobenzylammonio derivative to the monoclonal antibody (mAb) were optimized. The labeling yield was about 90% and the overall conjugation yield was 55-60%. The in vitro stability of the radio-iodinated mAb was good under physiological and non-physiological conditions. The immunoreactivity of the labeled mAb (SK-BR-3 cells) was retained in the one-pot two-step labeling. Negatively-charged polyhedral boron clusters can be used for indirect radio-iodination of mAbs.
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ISSN:0250-7005