Diabetic dyslipidemia and exercise affect coronary tone and differential regulation of conduit and microvessel K super(+) current

• Published in: American journal of physiology. Heart and circulatory physiology Vol. 288; no. 3; pp. H1233 - H1241
• Main Authors: Mokelke, E A, Dietz, N J, Eckman, D M, Nelson, M T, Sturek, M
• Format: Journal Article
• Language: English
• Published: 01.03.2005
• ISSN: 0363-6135; 1522-1539

Spontaneous transient outward K super(+) currents (STOCs) elicited by Ca super(2+) sparks and steady-state K super(+) currents modulate vascular reactivity, but effects of artery size, diabetic dyslipidemia, and exercise on these differentially regulated K super(+) currents are unclear. We studied the conduit arteries and microvessels of male Yucatan swine assigned to one of three groups for 20 wk: control (C, n = 7), diabetic dyslipidemic (DD, n = 6), or treadmill- trained DD animals (DDX, n = 7). Circumflex artery blood flow velocity obtained with intracoronary Doppler and lumen diameters obtained by intravascular ultrasound enabled calculation of absolute coronary blood flow (CBF). Ca super(2+) sparks were determined in pressurized microvessels, and perforated patch clamp assessed K super(+) current in smooth muscle cells isolated from conduits and microvessels. Baseline CBF in DD was decreased versus C. In pressurized microvessels, Ca super(2+) spark activity was significantly lower in DD versus C and DDX (P < 0.05 vs. DDX). STOCs were pronounced in microvessel ([approx]35 STOCs/min) in sharp contrast to conduit cells ([approx]2 STOCs/min). STOCs were decreased by 86% in DD versus C and DDX in microvessels; in contrast, there was no difference in STOCs across groups in conduit cells. Steady-state K super(+) current in microvessels was decreased in DD and DDX versus C; in contrast, steady-state K super(+) current in conduit cells was decreased in DDX versus DD and C. We conclude that steady-state K super(+) current and STOCs are differentially regulated in conduit versus microvessels in health and diabetic dyslipidemia. Exercise prevented diabetic dyslipidemia-induced decreases in baseline CBF, possibly via STOC-regulated basal microvascular tone.