Herpes Simplex Virus 1 DNA Packaging Proteins Encoded by U sub(L)6, U sub(L)15, U sub(L)17, U sub(L)28, and U sub(L)33 Are Located on the External Surface of the Viral Capsid

• Published in: Journal of virology Vol. 80; no. 21; pp. 10894 - 10899
• Main Authors: Wills, Elizabeth, Scholtes, Luella, Baines, Joel D
• Format: Journal Article
• Language: English
• Published: 01.11.2006
• Subjects: Herpes simplex virus 1
• ISSN: 0022-538X; 1098-5514

Studies to localize the herpes simplex virus 1 portal protein encoded by U sub(L)6, the putative terminase components encoded by U sub(L)15, U sub(L) 28, and U sub(L)33, the minor capsid proteins encoded by U sub(L)17, and the major scaffold protein ICP35 were conducted. ICP35 in B capsids was more resistant to trypsin digestion of intact capsids than pU sub(L)6, pU sub(L)15, pU sub(L)17, pU sub(L)28, or pU sub(L)33. ICP35 required sectioning of otherwise intact embedded capsids for immunoreactivity, whereas embedding and/or sectioning decreased the immunoreactivities of pU sub(L)6, pU sub(L)17, pU sub(L)28, and pU sub(L)33. Epitopes of pU sub(L)15 were recognized roughly equally well in both sectioned and unsectioned capsids. These data indicate that pU sub(L)6, pU sub(L)17, pU sub(L)28, pU sub(L)33, and at least some portion of pU sub(L)15 are located at the external surface of the capsid.