Baculovirus surface display of E super(r) super(n) super(s) envelope glycoprotein of classical swine fever virus

• Published in: Journal of virological methods Vol. 153; no. 2; pp. 149 - 155
• Main Authors: Xu, X G, Chiou, M T, Zhang, Y M, Tong, D W, Hu, J H, Zhang, M T, Liu, HJ
• Format: Journal Article
• Language: English
• Published: 01.11.2008
• Subjects: Baculovirus; Classical swine fever virus
• ISSN: 0166-0934
• DOI: 10.1016/j.jviromet.2008.07.019

Classical swine fever virus (CSFV) causes significant losses in the pig industry in many countries. E super(r) super(n) super(s) is an envelope glycoprotein of CSFV which is known to induce virus-neutralizing antibodies and protective immunity in the natural host. In this study, one recombinant baculoviruses BacSC-E super(r) super(n) super(s) expressing histidine-tagged E super(r) super(n) super(s) with the transmembrane domain (TM) and cytoplasmic domain (CTD) derived from baculovirus envelope protein gp64 was constructed and its immunizing efficacy was evaluated in a mouse model. After infection, E super(r) super(n) super(s) was expressed and anchored on the plasma membrane of Sf-9 cells, as demonstrated by Western-blot and confocal microscopy. Immunogold electron microscopy demonstrated that the E super(r) super(n) super(s) glycoprotein was successfully displayed on the baculoviral envelope. Vaccine tests in animals showed that BacSC-E super(r) super(n) super(s) elicited significantly higher E super(r) super(n) super(s) antibody titers in the immunized mouse models than the control group. This demonstrates that the BacSC-E super(r) super(n) super(s) vaccine can be used potentially against CSFV infections. This is the first report demonstrating the potential of E super(r) super(n) super(s)-pseudotyped baculovirus as a CSFV vaccine.