Response of NAD(P)H dehydrogenase complex to the alteration of CO sub(2) concentration in the cyanobacterium Synechocystis PCC6803

An NADPH-specific NDH-1 sub-complex was separated by native-polyacrylamide gel electrophoresis and detected by activity staining from the whole cell extracts of Synechocystis PCC6803. Low CO sub(2) caused an increase in the activity of this sub-complex quickly, accompanied by an evident increase in...

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Bibliographic Details
Published inJournal of plant physiology Vol. 160; no. 8; pp. 967 - 970
Main Authors Deng, Yong, Ye, Jiyu, Mi, Hualing, Shen, Yunkang
Format Journal Article
LanguageEnglish
Published 01.08.2003
Subjects
Cyanophyta
NADPH dehydrogenase
NdhK protein
Synechocystis
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Summary:An NADPH-specific NDH-1 sub-complex was separated by native-polyacrylamide gel electrophoresis and detected by activity staining from the whole cell extracts of Synechocystis PCC6803. Low CO sub(2) caused an increase in the activity of this sub-complex quickly, accompanied by an evident increase in the expression of NdhK and PSI-driven NADPH oxidation activity that can reflect the activity of NDH-1-mediated cyclic electron transport. During incubation with high CO sub(2), the activities of NDH-1 sub-complex and PSI-driven NADPH oxidation as well as the protein level of NdhK slightly increased at the beginning, but decreased evidently in various degrees along with incubation time. These results suggest that CO sub(2) concentration in vitro as a signal can control the activity of NDH-1 complex, and NDH-1 complex may in turn function in the regulation of CO sub(2) uptake.
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ISSN:0176-1617