Effect of Dicer1-deficient Liver on Metabolism and Transport Function of Bile Acid

• Published in: Progress In Modern Biomedicine Vol. 16; no. 29
• Main Authors: Sun, Xiao-xu, Yan, Hong, Liu, Ming-xia, Qiu, Ming-feng, Qi, Xin-ming, Ren, Jin
• Format: Journal Article
• Language: Chinese
• Published: 01.01.2016
• ISSN: 1673-6273

Objective:MicroRNA(miRNA) plays a pivotal role in cholesterol synthesis,metabolism and transport,but the role of miRNAs in bile acid metabolism and transport is unclear.Dicer is the key enzyme of miRNA biogenesis.This study focused on the impact of Dicer1 knockout on bile acid metabolism and transport in C57BL/6 mice.Methods:Cre recombinase driven by Albumin promoter and the floxed dicer allele were used for Dicer1 knockout in the liver of mice.Cobas biochemical analyzer was used to test bile acid level in the blood and liver; Real-time quantitative PCR was used to analyze gene expression.Results:In Dicer1 knockout mice,bile acids accumulated in the blood and liver,diffuse vacuolation of hepatocytes was observed,the single cell necrosis was found occasionally.Bile acid synthesis gene expression was slightly increased but without statistical difference; Oatplal,a bile acid uptake transporter,was obviously decreased in the dicer1 knockout mice.No obvious change can be seen in the expression of Ntcp and Oatp1b2;