Up-Regulation of Integrins alpha 2 beta 1 and alpha 2 beta 1 Expression in Human Foreskin Fibroblast Cells after In-Vitro Infection with Herpes Simplex Virus Type 1
The interaction of Herpes Simplex Virus type 1 (HSV-1) with human fetal foreskin fibroblast (HFFF) cell was studied using a recent isolate of HSV-1 which was propagated in Hep-2 cells. HFFF cells were challenged with HSV-1 with a multiplicity of infection (MOI) of 1 virus/cell for 24 hours. Flow cyt...
Saved in:
Published in | Iranian biomedical journal Vol. 5; no. 2-3; pp. 55 - 59 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
01.07.2001
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The interaction of Herpes Simplex Virus type 1 (HSV-1) with human fetal foreskin fibroblast (HFFF) cell was studied using a recent isolate of HSV-1 which was propagated in Hep-2 cells. HFFF cells were challenged with HSV-1 with a multiplicity of infection (MOI) of 1 virus/cell for 24 hours. Flow cytometric analysis demonstrated that HSV-1 challenged HFFF cells expressed increased levels of alpha 2 beta 1, alpha 3 beta 1 (a collagen and laminin receptor) and LFA-1 (a beta 2 integrin, ligand for intercellular adhesion molecule-1, ICAM-1) integrins without a significant change in the expression of alpha 5 beta 1 (a fibronectin receptor) as compared to the mock-challenged cells. In addition, virus challenged HFFF cells showed increased expression of ICAM-1 and HLA class I antigen when compared to mock-infected cells. These results suggest that HSV-1 is capable of inducing functional changes in human foreskin fibroblast cells. The altered expression of cell-matrix and cell-cell adhesion molecules resulting from HSV-1 infection could contribute to the immunopathological diseases associated with HSV-1 infection in vivo. |
---|---|
Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-1 |
ISSN: | 1028-852X |