Multiresidue method for simultaneous analysis of aflatoxin M sub(1), avermectins, organophosphate pesticides and milbemycin in milk by ultra-performance liquid chromatography coupled to tandem mass spectrometry

• Published in: Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment Vol. 33; no. 6; pp. 995 - 1002
• Main Authors: dos Anjos, Marianna Ramos, Castro, Izabela Miranda de, Souza, Maria de Lourdes Mendes de, de Lima, Virginia Veronica, de Aquino-Neto, Francisco Radler
• Format: Journal Article
• Language: English
• Published: 02.06.2016
• ISSN: 1944-0049; 1944-0057
• DOI: 10.1080/19440049.2016.1175227

A method developed for the simultaneous analysis of aflatoxin M sub(1), abamectin, doramectin, eprinomectin, ivermectin, moxidectin, acephate, azinphos-ethyl, azinphos-methyl, diazinon, methamidophos, methidathion, mevinphos, pirimiphos-ethyl and pirimiphos-methyl in whole raw milk, based on the QuEChERS method for extraction and clean-up, with detection and quantification by ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) is described. The method was validated according to parameters of the Analytical Quality Assurance Manual from the Brazilian Ministry of Agriculture and Commission Decision 2002/657/EC, and proved suitable for analysis of these analytes within the proposed working range, with recovery values between 77% and 110%, a standard deviation lower than 20%, limits of detection between 0.05 and 0.99 mu g l super(-) super(1), and limits of quantification between 0.15 and 1.98 mu g l super(-1). Samples from animals treated with abamectin, doramectin, ivermectin and diazinon were analysed by the validated method. Residues of aflatoxin M sub(1) were also found in field samples at levels below the established maximum residue limit.