PS-301Conditions Of Intestinal Colonisation In Preterm Infants During The First Month Of Life

• Published in: Archives of disease in childhood Vol. 99; no. Suppl 2; p. A220
• Main Authors: Martin Alvarez, E, Zamorano-Bonilla, L, Jimenez-Cabanillas, M V, Serrano-Lopez, L, Pena-Caballero, M, Hurtado-Suazo, JA
• Format: Journal Article
• Language: English
• Published: 01.10.2014
• Subjects: Bacteroides; Bifidobacterium; Clostridium; Escherichia coli; Lactobacillus
• ISSN: 0003-9888
• DOI: 10.1136/archdischild-2014-307384.600

AimTo describe intestinal microbiota in preterm infants admitted to the Neonatal Unit using molecular techniques, and assess the impact of different conditions.Methods/study designA two year period Descriptive study (2011-2012) of gut microbiota in stools from newborns borns under 35 week gestational age (WGA) at birth, admitted in a Neonatal Care Unit. Stool samples were collected: M1 (meconium), M2 (first week), M3 (first month). 5 groups of bacteria were analysed using qPCR technique: Bacteroides, Bifidobacterium, Escherichia coli, Clostridiums and Lactobacillus. Maternal, perinatal and neonate variables were registered. Statistic programs SPSSvs 20.ResultsAbstract PS-301 Table 1 E coli Clostridium Bacteroides Bifidobacterium Lactobacillus M1 (n = 82)1,51 103 47,322,534,16 104 3,30 103 M2 (n = 74)4,77 103 31,6312,392,04 105 7,04 103 M3 (n = 35)1,14 104 43,0432,264,98 106 8,39 103 Value: p50 cfu/gIn the first month a marked increase (x120) in Bifidobacterium is observed. The increase of Lactobacillus (x2) and E. coli (x7) is lower. Bacteroides and Clostridium remain stable.Regarding WGA in less than or equal to 30 vs > 30 we observed:- Bifidobacterial colonisation is delayed at birth (2,35 104 vs 6,02 104 cfu/g; p = 0,09) and at one week birth age (3,98 104 vs 1,49 106 cfu/g; p = 0,007).- Higher numbers of E. coli from the first sample (4,37 103 vs 9,07 102 cfu/g; 1,55 104 vs 2,93 103 cfu/g; 2,94 104 vs 9,69 103 cfu/g), although no significant statistically differences were detected.ConclusionsThe number of Bifidobacterium and Lactobacillus in faecal samples is higher than the content in Bacteroides, Clostridium and E.coli. The colonisation process of studied bacteria is delayed in prematures born at lower WGA, except for E. coli.