Mn(ii) tags for DEER distance measurements in proteins viaC-S attachment

Mn super(2+) chelating tags for Mn super(2+)-Mn super(2+) distance measurements by pulse EPR spectroscopy were developed. They feature a stable C-S conjugation to the protein, high reactivity towards cysteine thiols and short and rigid linkers that can be used in distance measurements with high reso...

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Published inDalton transactions : an international journal of inorganic chemistry Vol. 44; no. 48; pp. 20812 - 20816
Main Authors Martorana, Andrea, Yang, Yin, Zhao, Yu, Li, Qing-Feng, Su, Xun-Cheng, Goldfarb, Daniella
Format Journal Article
LanguageEnglish
Published 01.12.2015
Subjects
Attachment
Chelating
Conjugation
Cysteine
Deer
Proteins
Spectroscopy
Tags
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Summary:Mn super(2+) chelating tags for Mn super(2+)-Mn super(2+) distance measurements by pulse EPR spectroscopy were developed. They feature a stable C-S conjugation to the protein, high reactivity towards cysteine thiols and short and rigid linkers that can be used in distance measurements with high resolution under reductive conditions. Double electron-electron resonance measurements at 95 GHz on ubiquitin labeled with these tags showed the expected narrow distance distribution.
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ISSN:1477-9226
1477-9234
DOI:10.1039/c5dt04123f