Diversity of Scenedesmus from Itasca State Park, Minnesota

The diversity of Scenedesmus and Scenedesmus-like taxa from Itasca State Park, Minnesota was assessed using light microscopy and molecular techniques. Thirty isolates from various ponds and lakes in Itasca State Park were examined. Light microscopy showed many similarities in morphology among isolat...

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Bibliographic Details
Published inJournal of phycology Vol. 39; no. S1; p. 26
Main Authors Johnson, J L, Fawley, M W, Fawley, K P
Format Journal Article
LanguageEnglish
Published 01.06.2003
Subjects
Chlorophyta
Freshwater
Scenedesmus
USA, Minnesota, Itasca State Park
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Summary:The diversity of Scenedesmus and Scenedesmus-like taxa from Itasca State Park, Minnesota was assessed using light microscopy and molecular techniques. Thirty isolates from various ponds and lakes in Itasca State Park were examined. Light microscopy showed many similarities in morphology among isolates, but PCR-RFLP analysis of the ribosomal ITS region from these isolates revealed twenty different types. A previous study from Itasca State Park using only light microscopy found only six taxa of Scenedesmus; however, our results suggest that there is much greater diversity than previously suspected. DNA sequences of the 5.8S ribosomal subunit and the ITS-2 region from our isolates are presently being determined and will be used to assess this diversity in greater detail.
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ISSN:0022-3646
DOI:10.1111/j.0022-3646.2003.03906001_74.x