T Cells in G sub(1) Provide a Memory-Like Response to Secondary Stimulation

• Published in: The Journal of immunology (1950) Vol. 174; no. 7; pp. 4010 - 4018
• Main Authors: Munitic, Ivana, Ryan, Philip E, Ashwell, Jonathan D
• Format: Journal Article
• Language: English
• Published: 01.04.2005
• ISSN: 0022-1767

The commitment of naive T cells to proliferate is a function of the strength and duration of stimuli mediated by the TCR and coreceptors. Ranges of 2-20 h of stimulation have been reported as necessary in vitro. Whether T cells actually experience uninterrupted stimulation for such long periods under physiological conditions is controversial. Here we ask whether commitment to proliferate requires continuous stimulation, or can T cells integrate intermittent periods of stimulation. T cells were stimulated for two short-term (subthreshold) periods (5-7 h) either sequentially or separated by an interval of rest. Naive lymph node T cells were able to integrate interrupted stimulation, even when the duration of rest was as long as 2 days. Furthermore, when short-term-stimulated T cells were separated by density, three populations were observed: low density blasts, intermediate density G sub(1) cells, and high density G sub(0) cells. Low density cells progressed to division without further stimulation, whereas G sub(0) and G sub(1) cells remained undivided. However, after a period of rest, a second subthreshold stimulation caused the G sub(1) but not the G sub(0) fraction to quickly proceed through the cell cycle. We conclude that noncycling T cells in the G sub(1) phase of the cell cycle remain in a state of readiness for prolonged periods of time, and may represent a population of memory-like effectors capable of responding rapidly to antigenic challenge.