Multiple beta sub(1) Integrins Mediate Enhancement of Human Airway Smooth Muscle Cytokine Secretion by Fibronectin and Type I Collagen

• Published in: The Journal of immunology (1950) Vol. 174; no. 4; pp. 2258 - 2264
• Main Authors: Peng, Qi, Lai, Dilys, Nguyen, Trang T-B, Chan, Vivien, Matsuda, Takeshi, Hirst, Stuart J
• Format: Journal Article
• Language: English
• Published: 15.02.2005
• ISSN: 0022-1767

Altered airway smooth muscle (ASM) function and enrichment of the extracellular matrix (ECM) with interstitial collagen and fibronectin are major pathological features of airway remodeling in asthma. We have previously shown that these ECM components confer enhanced ASM proliferation in vitro, but their action on its newly characterized secretory function is unknown. Here, we examined the effects of fibronectin and collagen types I III, and V on IL-1 beta - dependent secretory responses of human ASM cells, and characterized the involvement of specific integrins. Cytokine production (eotaxin, RANTES, and GM- CSF) was evaluated by ELISA, RT-PCR, and flow cytometry. Function-blocking integrin mAbs and RGD (Arg-Gly-Asp)-blocking peptides were used to identify integrin involvement. IL-1 beta -dependent release of eotaxin, RANTES, and GM-CSF was enhanced by fibronectin and by fibrillar and monomeric type I collagen, with similar changes in mRNA abundance. Collagen types III and V had no effect on eotaxin or RANTES release but did modulate GM-CSF. Analogous changes in intracellular cytokine accumulation were found, but in <25% of the total ASM cell population. Function-blocking Ab and RGD peptide studies revealed that alpha sub(2) beta sub(1), alpha sub(5) beta sub(1), alpha sub(v) beta sub(1), and alpha sub(v) beta sub(3) integrins were required for up-regulation of IL-1 beta -dependent ASM secretory responses by fibronectin, while alpha sub(2) beta sub(1) was an important transducer for type I collagen. Thus, fibronectin and type I collagen enhance IL-1 beta -dependent ASM secretory responses through a beta sub(1) integrin-dependent mechanism. Enhancement of cytokine release from ASM by these ECM components may contribute to airway wall inflammation and remodeling in asthma.