Rare HLA alleles in Czech hematological patients and their HSC unrelated donors

• Published in: Genes and immunity Vol. 6; p. S74
• Main Authors: Dobrovolna, M, Vrana, M, Matejkova, E
• Format: Journal Article
• Language: English
• Published: 01.04.2005
• Subjects: Czech Rep
• ISSN: 1466-4879

It has been demonstrated that even a single amino acid difference in HLA complex between donor and recipient can affect an outcome of hematopoietic stem cell transplantation (HSCT). In the period of 2002-2004 we examined a group of 396 Czech patients potentially indicated to the HSCT and their 317 unrealted donors. HLA loci -A, -B, -C, -DRB1, -DQB1 were typed using PCR-SSP on high resolution level. Rare alleles (with frequency <0.01) in loci HLA-A and -B were confirmed by direct sequencing. Three HLA-A (A*0217, A*3004, A*6901) and six HLA-B (B*0703, B*0705, B*4006, B*4047, B*4427, B*5702) rare alleles were detected in this cohort. Peptides coded by B*0705 and B*4427 are different from their common equivalents (*0702 and *4402) only in one amino acid, the others differ more. All together 14 patients carried rare allele (HLA-A 3 patients and HLA-B 11 patients), matched donor has been found for 8 of them.