Cytochrome c sub(3) mutants of Desulfovibrio desulfuricans

To explore the physiological role of tetraheme cytochrome c sub(3) in the sulfate-reducing bacterium Desulfovibrio desulfuricans G20, the gene encoding the preapoprotein was cloned, sequenced, and mutated by plasmid insertion. The physical analysis of the DNA from the strain carrying the integrated...

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Published inApplied and environmental microbiology Vol. 66; no. 2; pp. 671 - 677
Main Authors Rapp-Giles, B J, Casalot, L, English, R S, Ringbauer, JA Jr, Dolla, A, Wall, J D
Format Journal Article
LanguageEnglish
Published 01.02.2000
Subjects
Desulfovibrio desulfuricans
Lactic acid
Pyruvic acid
Sulfate
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Summary:To explore the physiological role of tetraheme cytochrome c sub(3) in the sulfate-reducing bacterium Desulfovibrio desulfuricans G20, the gene encoding the preapoprotein was cloned, sequenced, and mutated by plasmid insertion. The physical analysis of the DNA from the strain carrying the integrated plasmid showed that the insertion was successful. The growth rate of the mutant on lactate with sulfate was comparable to that of the wild type; however, mutant cultures did not achieve the same cell densities. Pyruvate, the oxidation product of lactate, served as a poor electron source for the mutant. Unexpectedly, the mutant was able to grow on hydrogen-sulfate medium. These data support a role for tetraheme cytochrome c sub(3) in the electron transport pathway from pyruvate to sulfate or sulfite in D. desulfuricans G20.
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ISSN:0099-2240
DOI:10.1128/AEM.66.2.671-677.2000