Seroprevalence for brucellosis and leptospirosis in dogs from Belem and Castanhal, State of Para, Brazil

• Published in: Acta amazonica Vol. 45; no. 3; pp. 265 - 270
• Main Authors: da Paz, Giselle Souza, de Souza Rocha, Katarine, de Souza Lima, Michele, Jorge, Ediene Moura, Pantoja, Jose Carlos Figueiredo, de Moraes, Carla Cristina Guimaraes, Langoni, Helio
• Format: Journal Article
• Language: Portuguese
• Published: 01.09.2015
• ISSN: 0044-5967; 1809-4392
• DOI: 10.1590/1809-4392201403486

Brucellosis and leptospirosis are widely spread bacterial infections and dogs are the most important source of infection and reservoir for diseases. Dogs can disseminate the agents in the environment and transmit them to humans and/or other animals. The objective of this study was assess the occurrence of reactive to antibodies anti-Leptospira spp., Brucella canis and B. abortus in Belem and Castanhal, State of Para, Amazon, Brazil. A total of 156 samples were randomly collected in the city of Belem and 158 samples in Castanhal. The anti-B. canis antibodies research was performed by Agar Gel Immunodiffusion (AGID) with and without 2-mercaptoethanol serum treatment (AGID-2ME).To assess the anti-B. abortus antibodies, the technique of Fast Seroagglutination with buffered acidified plate antigen (BAPAT) was used. For anti-Leptospira spp. antibodies research, the Microscopic Agglutination Technique (MAT) was used. No animal reacted to Brucella abortus and one animal was reactive to B. canis at the AGID, but it was negative to the AGID-2ME test. Seventeen percent of dogs (47/274) presented anti-Leptospira spp. antibodies, with prevalence of serovar Canicola. The dogs from Belem and Castanhal are not source of infection for 5. abortus and B. canis, however, they are reservoirs for different serovars of Leptospira spp.Original Abstract: Brucelose e leptospirose sao enfermidades bacterianas ampiamente disseminadas e o cao e importante fonte de infeccao e reservatorio para ambas, podendo eliminar o agente no meio ambiente, e transmiti-lo para humanos e/ou outros animals. O presente estudo teve como objetivo pesquisar a ocorrencia de caes reagentes para anticorpos contra Leptospira spp., Brucella canis e B. abortus em Belem e Castanhal, Para, Amazonia, Brasil. Foram colhidas de forma aleatoria 156 amostras no municipio de Belem e 158 amostras em Castanhal. A pesquisa de anticorpos anti-B. canis foi realizada pela tecnica de Imunodifusao em Gel de Agar (IDGA) com e sem tratamento do soro com 2-mercaptoetanol (IDGA-2ME) e para pesquisa de anticorpos anti-B. abortus foi utilizada a tecnica de Soroaglutinacao Rapida com antigeno acidificado tampo nado (AAT). Para pesquisa de anticorpos contra-Leptospira spp. utilizou-se a Tecnica de Aglutinacao Microscopica (MAT). Nenhum animal reagiu para Brucella abortus e um animal foi reagente para B. canis na IDGA, porem foi negativo na IDGA-2ME. Dezessete por cento dos caes (47/274) apresentaram anticorpos contra-Leptospira spp., com predominancia do sorovar Canicola. Os caes de Belem e Castanhal nao sao fontes de infeccao para B. abortus e B. canis, no entanto sao reservatorios de diferentes sorovares de Leptospira spp.