Trp1, a Candidate Protein for the Store-operated Ca super(2+) Influx Mechanism in Salivary Gland Cells
The trp gene family has been proposed to encode the store-operated Ca super(2+) influx (SOC) channel(s). This study examines the role of Trp1 in the SOC mechanism of salivary gland cells. htrp1, htrp3, and Trp1 were detected in the human submandibular gland cell line (HSG). HSG cells stably transfec...
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Published in | The Journal of biological chemistry Vol. 275; no. 5; pp. 3403 - 3411 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
04.02.2000
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Subjects | |
Online Access | Get full text |
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Summary: | The trp gene family has been proposed to encode the store-operated Ca super(2+) influx (SOC) channel(s). This study examines the role of Trp1 in the SOC mechanism of salivary gland cells. htrp1, htrp3, and Trp1 were detected in the human submandibular gland cell line (HSG). HSG cells stably transfected with htrp1 alpha cDNA displayed (i) a higher level of Trp1, (ii) a 3-5-fold increase in SOC (thapsigargin-stimulated Ca super(2+) influx), determined by [Ca super(2+)]i and Ca super(2+)-activated K super(+) channel current measurements, and (iii) similar basal Ca super(2+) permeability, and inhibition of SOC by Gd super(3+) but not by Zn super(2+), as compared with control cells. Importantly, (i) transfection of HSG cells with antisense trp1 alpha cDNA decreased endogenous [108qlevel and significantly attenuated SOC, and (ii) transfection of HSG cells with htrp3 cDNA did not increase SOC. These data demonstrate an association between Trp1 and SOC and strongly suggest that Trp1 is involved in this mechanism in HSG cells. Consistent with this suggestion, Trp1 was detected in the plasma membrane region, the proposed site of SOC, of acinar and ductal cells in intact rat submandibular glands. Based on these aggregate data, we propose Trp1 as a candidate protein for the SOC mechanism in salivary gland cells. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-1 |
ISSN: | 0021-9258 |
DOI: | 10.1074/jbc.275.5.3403 |