Identification of sigma super(B)-Dependent Genes in Bacillus subtilis Using a Promoter Consensus-Directed Search and Oligonucleotide Hybridization

A consensus-directed search for sigma super(B) promoters was used to locate potential candidates for new sigma super(B)-dependent genes in Bacillus subtilis. Screening of those candidates by oligonucleotide hybridizations with total RNA from exponentially growing or ethanol-stressed cells of the wil...

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Published inJournal of bacteriology Vol. 181; no. 18; pp. 5718 - 5724
Main Authors Petersohn, A, Bernhardt, J, Gerth, U, Hoeper, D, Koburger, T, Voelker, U, Hecker, M
Format Journal Article
LanguageEnglish
Published 01.09.1999
Subjects
Bacillus subtilis
dehydrogenase
s@uB Factor
sigB gene
thioredoxin
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Summary:A consensus-directed search for sigma super(B) promoters was used to locate potential candidates for new sigma super(B)-dependent genes in Bacillus subtilis. Screening of those candidates by oligonucleotide hybridizations with total RNA from exponentially growing or ethanol-stressed cells of the wild type as well as a sigB mutant revealed 22 genes that required sigma super(B) for induction by ethanol. Although almost 50% of the proteins encoded by the newly discovered sigma super(B)-dependent stress genes seem to be membrane localized, biochemical functions have so far not been defined for any of the gene products. Allocation of the genes to the sigma super(B)-dependent stress regulon may indicate a potential function in the establishment of a multiple stress resistance. AldY and YhdF show similarities to NAD(P)-dependent dehydrogenases and YdbP to thioredoxins, supporting our suggestion that sigma super(B)-dependent proteins may be involved in the maintenance of the intracellular redox balance after stress.
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ISSN:0021-9193