Identification of sigma super(B)-Dependent Genes in Bacillus subtilis Using a Promoter Consensus-Directed Search and Oligonucleotide Hybridization
A consensus-directed search for sigma super(B) promoters was used to locate potential candidates for new sigma super(B)-dependent genes in Bacillus subtilis. Screening of those candidates by oligonucleotide hybridizations with total RNA from exponentially growing or ethanol-stressed cells of the wil...
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Published in | Journal of bacteriology Vol. 181; no. 18; pp. 5718 - 5724 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
01.09.1999
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Subjects | |
Online Access | Get full text |
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Summary: | A consensus-directed search for sigma super(B) promoters was used to locate potential candidates for new sigma super(B)-dependent genes in Bacillus subtilis. Screening of those candidates by oligonucleotide hybridizations with total RNA from exponentially growing or ethanol-stressed cells of the wild type as well as a sigB mutant revealed 22 genes that required sigma super(B) for induction by ethanol. Although almost 50% of the proteins encoded by the newly discovered sigma super(B)-dependent stress genes seem to be membrane localized, biochemical functions have so far not been defined for any of the gene products. Allocation of the genes to the sigma super(B)-dependent stress regulon may indicate a potential function in the establishment of a multiple stress resistance. AldY and YhdF show similarities to NAD(P)-dependent dehydrogenases and YdbP to thioredoxins, supporting our suggestion that sigma super(B)-dependent proteins may be involved in the maintenance of the intracellular redox balance after stress. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-1 |
ISSN: | 0021-9193 |