New Antimicrobial Peptides from the Skin Secretion of the South American Frog Leptodactylus ocellatus (Anura: Leptodactylidae)

The emergence, in recent years, of microbial resistance to commonly used antibiotics has aroused a search for new naturally occurring bactericidal and fungicidal agents that may have clinical utility. In the present study, three new antimicrobial peptides were purified from the electrical-stimulated...

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Published inThe protein journal Vol. 23; no. 8; pp. 501 - 508
Main Authors Nascimento, ACC, Zanotta, L C, Kyaw, C M, Schwartz, ENF, Schwartz, CA, Sebben, A, Sousa, M V, Fontes, W, Castro, MS
Format Journal Article
LanguageEnglish
Published 02.11.2004
Subjects
Freshwater
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Summary:The emergence, in recent years, of microbial resistance to commonly used antibiotics has aroused a search for new naturally occurring bactericidal and fungicidal agents that may have clinical utility. In the present study, three new antimicrobial peptides were purified from the electrical-stimulated skin secretion of the South American frog Leptodactylus ocellatus by reversed-phase chromatographic procedures. Ocellatin 1 ( super(1)GVVDILKGAGKDLLAHLVG ISEKV super(25)-CONH sub(2)), ocellatin 2 ( super(1)GVVDILKGAGKDLLAHLVGKISEKV super(25)-CONH sub(2)) and ocellatin 3 ( super(1)GVLDILKNAAKNILAHAAEQI super(21)-CONH sub(2)) are structurally related peptides. These peptides present hemolytic activity against human erythrocytes and are also active against Escherichia coli. Ocellatins exhibit significant sequence similarity to other amphibian antimicrobial peptides, mainly to brevinin 2ED from Rana esculenta.
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ISSN:1572-3887